Infectious Disease and Pathogen Detection
Digital PCR for Infectious Diseases and Environmental Pathogens
For bacterial and viral pathogens, digital PCR affords higher sensitivity and accuracy for detecting low-level abundance nucleic acids, providing absolute quantification and high specificity. While qPCR has traditionally been the preferred technique used for such applications in pathogen detection, digital PCR affords better accuracy in the presence of inhibitors, in addition to getting absolute quantification without a standard curve.
Digital PCR can be used to for such applications as:
- Viral load – quantifying HIV or HPV in latent reservoirs
- SARS-CoV-2 detection -detecting variants at both the population and the patient levels
- Food-borne pathogens – determining the presence and quantification of microbial pathogens for food safety applications
From sample extraction to analysis for pathogen detection
The Nio+ system offers a fast, easy-to-use platform for quantification of genetic targets. In order to detect and quantify multiple genetic targets in a single run, the Nio+ system also offers the highest multiplexing available on the market today. When coupled with a sample preparation platform such as the Maxwell® RSC instruments from Promega, users are provided with a seamless end-to-end workflow – from sample preparation to analysis.
Digital PCR for pathogen detection
Stilla provides digital PCR kits out-of-the-box, as well as custom assay design services in order to help researchers characterize and quantify genetic targets for applications detecting and quantifying low-level abundance bacterial and viral pathogens.
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A 3-color Crystal Digital PCR kit for detection of Covid-19
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3-color Crystal Digital PCR assays for HPV16 and HPV18 detection
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RNA quantification with Crystal RT-dPCR
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Absolute quantification of priority bacteria in aquaculture using digital PCR
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Identification of MRSA using 3-color digital PCR
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Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA
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Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities
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Simpler and faster Covid-19 testing: Strategies to streamline SARS-CoV-2 molecular assays
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Highly Sensitive Quantification of Plasma SARS-CoV-2 RNA Sheds Light on its Potential Clinical Value
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Generation and characterization of two strains of transgene mice expressing chimeric MiniSOG-MusPrP
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Cyprinid herpesvirus 3 Evolves In Vitro through an Assemblage of Haplotypes that Alternatively Become Dominant or Under-Represented
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IP10, KC and M-CSF is remarkably increased in the brains from the various strains of experimental mice infected with different scrapie agents
From custom assay design services to onsite training, Stilla has the knowledge and expertise to help researchers get started on their workflow. Stilla technologies has compiled several statistical tools to help get your started with your digital PCR assays, including:
For Research Use Only. Not for use in diagnostic procedures.